[Preparation and physicochemical properties of vinorelbine liposomes]

To prepare the vinorelbine liposomes and investigate their physicochemical properties.

Vinorelbine liposomes were prepared by thin-film ultrasonication method. The morphology of the liposomes was detected by transmission electron microscopy. The partical size distribution and zeta potential were investigated by laser scattering. Sephadex G-50 was applied to separate the free drug and liposomes in order to determine the entrapment efficiency and leakage efficiency of the liposomes. The release characteristics of vinorelbine liposomes were measured by dialysis method.

The particle size of the liposome was 149.2 nm, the bilayer of the liposome was observed obviously. The zeta potential was 38.62 mv. The entrapment efficiency of vinorelbine liposomes was more than 85% (n=3), and the leakage efficiency was less than 9.0% preserved in refrigerator ( <4 degrees C). In vitro release test, it could sustain for 24 h and conform to the Higuchi model.

The vinorelbine liposomes have uniform partical size, fine-looking, high entrapment efficiency and sustained releasing effect.