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Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
Published on: November 18, 2022
[Effects and mechanism of Plastrum testudinis extracts on PC12 apoptosis]
Yang Liu1, Yi-Ling Wu, Jia-Hui Cao
1Department of Anatomy, Preclinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Objective:
To observe the inhibitive effects of Plastrum testudinis Extracts (PTE) on 6-Hydroxydopamine (6-OHDA) induced PC12 cells apoptosis and explore its mechanism.
Methods:
PC12 apoptosis model was established by serum starvation and damaged for 24 hours. The cells were randomly divided into four groups:control group, 6-OHDA group, PTE 3, 30 microg/mL group. Cell optical density was determined by MTT; Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the BCL-X/L expression.
Results:
MTT and FCM analysis demonstrated that PTE can elevate PC12 cells viability and reduce their apoptotic ratio in a dose dependent manner. Western blot showed that PTE promoted the expression of BCL-X/L.
Conclusion:
PTE can inhibit the apoptosis of PC12 induced by 6-OHDA in a dose dependent manner, and its mechanism maybe associated partially with up-regulating BCL-X/L signaling pathway.
