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[Determination of ergosterol in Ganoderma lucidum from different varieties and cultured tree species by HPLC]
Jing-jing Liu1, Wen-hua Huang, Ming-liang Lv
1Chinese Academy of Medical Sciences/Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100193, China. jing_jing_6@163.com
Objective:
HPLC method for determining ergosterol was established to evaluate the quality of Ganoderma lucidum and to analyze of the ergosterol in different varieties and cultrued tree species.
Methods:
Samples from 17 different varieties and 14 different tree species were quantified by HPLC. The RP-HPLC was conducted on Diamonsil C18 column with acetonitrile as the mobile phase at 40 degrees C. The flow rate was 1.0 mL/min; and the detection wavelength was 282 nm.
Results:
The fluctuations of ergosterol content was 0.093%-0.243% among different varieties and 0.080%-0.227% among different tree species. Hanzhi-2, Yuanzhi, Ming-1, Yesheng-1 hao were excellent at both ergosterol content and conversion rate among all the varieties, and Bai Li, Yang Mei have comparatively more ergosterol and high conversion rate among all the tree species in this study.
Conclusions:
This method is simple, sensitive and accurate. It could be used to determine the contents of ergosterol and the evaluation of the quality in Ganoderma. It also provides references for choosing varieties and tree species when culture Ganoderma.
