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Developing visible fluorogenic 'click-on' dyes for cellular imaging
Jianjun Qi1, Myung-Shin Han, Yu-Cheng Chang
1Department of Radiology, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.
Abstract:
New fluorogenic dyes were designed and synthesized based on Cu(I)-catalyzed 'click' reaction. Conjugating weakly fluorescent benzothiazole derivatives with an electron-deficient alkyne group at the 2-position with azide-containing molecules in aqueous solution form 'click-on' fluorescent adducts. Model reactions and cell culture experiment indicated that the developed 'click-on' dye could be applied to labeling various biomolecules, such as nucleic acids, proteins, and other molecules, in cells.
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