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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: May 30, 2026

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
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Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Published on: January 19, 2024

Developing visible fluorogenic 'click-on' dyes for cellular imaging.

Jianjun Qi1, Myung-Shin Han, Yu-Cheng Chang

  • 1Department of Radiology, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.

Bioconjugate Chemistry
|August 10, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed new

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Related Experiment Videos

Last Updated: May 30, 2026

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection
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Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Published on: January 19, 2024

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Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging

Published on: March 15, 2010

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Published on: July 6, 2016

Area of Science:

  • Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Development of novel fluorogenic probes is crucial for advanced biological imaging.
  • Copper(I)-catalyzed click chemistry offers a reliable method for bioconjugation.

Purpose of the Study:

  • To design and synthesize novel fluorogenic dyes utilizing the click reaction.
  • To evaluate the utility of these dyes for labeling biomolecules in cellular environments.

Main Methods:

  • Synthesis of benzothiazole-based fluorogenic dyes with an alkyne group.
  • Cu(I)-catalyzed azide-alkyne cycloaddition (click reaction) in aqueous media.
  • Cell culture experiments for biomolecule labeling and imaging.

Main Results:

  • Successfully synthesized novel 'click-on' fluorogenic dyes.
  • Demonstrated efficient conjugation of dyes with azide-containing biomolecules.
  • Validated the dye's applicability for labeling nucleic acids, proteins, and other molecules within cells.

Conclusions:

  • The developed click-on dyes are effective tools for bioimaging.
  • This approach enables versatile labeling of diverse biomolecules in live cells.
  • The click chemistry-based strategy provides a robust platform for fluorescent probe development.