Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Interference and Diffraction02:18

Interference and Diffraction

Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Nanoscale Spatial Heterogeneity in a Perovskite-to-Brownmillerite Topotactic Phase Transformation.

ACS applied materials & interfaces·2026
Same author

Efficacy and Safety of Pregabalin and Alpha-Lipoic Acid Combination in Patients With Painful Diabetic Peripheral Neuropathy: A Randomized, Open-Label, Non-Inferiority, Phase IV Clinical Trial and Subgroup Analysis (OPTIMUM Study).

Diabetes, obesity & metabolism·2026
Same author

Clinical utility of 1-h post-load glucose in accelerating diagnosis of type 2 diabetes: a prospective cohort study.

Acta diabetologica·2026
Same author

Optically active defect states and valley depolarization in monolayer MoS<sub>2</sub> induced by high-energy electron-beam irradiation.

Nanoscale·2026
Same author

Tripotent Lgr5-positive Progenitor Stem Cells Co-exist With More Primitive, Pluripotent and Quiescent VSELs.

Stem cell reviews and reports·2026
Same author

A novel disposition index without insulin is an earlier and sensitive predictor of type 2 diabetes than current diagnostic criteria.

Diabetes research and clinical practice·2026
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
06:49

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Published on: August 6, 2008

Dichroic coherent diffractive imaging.

Ashish Tripathi1, Jyoti Mohanty, Sebastian H Dietze

  • 1Department of Physics, University of California at San Diego, La Jolla, CA 92093, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 10, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a new lensless X-ray imaging technique to map magnetic nanostructures. This method overcomes limitations of traditional microscopes, enabling detailed visualization of magnetic domains for advanced materials and technologies.

More Related Videos

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Related Experiment Videos

Last Updated: May 30, 2026

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
06:49

Phase Contrast and Differential Interference Contrast (DIC) Microscopy

Published on: August 6, 2008

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Area of Science:

  • Condensed Matter Physics
  • Materials Science
  • Nanotechnology

Background:

  • Understanding nanoscale electronic structure is key to complex magnetic oxides and emergent phenomena.
  • Advanced probes are needed for nanometer length and subpicosecond time-scale investigations.
  • Polarized X-rays offer elemental and magnetic specificity but traditional microscopes have resolution limits.

Purpose of the Study:

  • To present a novel lensless imaging approach for mapping magnetic nanostructures.
  • To overcome the resolution limitations imposed by X-ray optics fabrication in traditional microscopes.
  • To enable imaging of magnetic domain configurations in extended nanostructures.

Main Methods:

  • A novel lensless imaging technique using scanned dichroic coherent diffraction patterns.
  • Numerical inversion of diffraction data to reconstruct magnetic domain configuration.
  • Demonstration on a gadolinium/iron (Gd/Fe) multilayer with perpendicular anisotropy.

Main Results:

  • Successfully mapped ferrimagnetic labyrinthine domains in a Gd/Fe multilayer.
  • Visualized the evolution of magnetic domain structure through a magnetization hysteresis loop.
  • The method is scalable to diffraction-limited resolution and applicable to large samples.

Conclusions:

  • The developed lensless X-ray imaging technique provides a powerful tool for nanoscale magnetic structure analysis.
  • This approach bypasses the need for precision optics and reference waves, unlike holographic methods.
  • It holds significant potential for advancing fundamental physics understanding and information technologies.