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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

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Related Experiment Video

Updated: May 30, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Published on: April 16, 2019

Silicalite nanoparticles that promote transgene expression.

Megan E Pearce1, Hoang Q Mai, Namhoon Lee

  • 1Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.

Nanotechnology
|August 10, 2011
PubMed
Summary
This summary is machine-generated.

New silicalite nanoparticles enhance gene delivery. Amine-functionalized nanoparticles significantly boost plasmid DNA (pDNA) transfection, while unmodified nanoparticles improve poly(ethylene imine)-pDNA complex delivery, showing promise for non-viral gene therapy.

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Last Updated: May 30, 2026

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
08:53

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Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Materials Science

Background:

  • Gene delivery systems are crucial for genetic therapies.
  • Non-viral vectors offer a safer alternative to viral vectors but often face efficiency challenges.
  • Poly(ethylene imine)-plasmid DNA (PEI-pDNA) complexes are widely studied non-viral gene delivery vectors.

Purpose of the Study:

  • To investigate the potential of silicalite nanoparticles as enhancers for gene transfection.
  • To evaluate the effect of surface functionalization (amine groups) on silicalite nanoparticle performance.
  • To assess the safety and cellular localization of these nanoparticles for gene delivery applications.

Main Methods:

  • Synthesis and characterization of silicalite nanoparticles (mean size 55 nm).
  • Surface functionalization of silicalite nanoparticles with amine groups and zeta potential measurements.
  • Transfection efficiency assays using plasmid DNA (pDNA) alone and PEI-pDNA complexes in HEK-293 cells.
  • Cellular localization studies and MTT assays to assess nanoparticle toxicity.

Main Results:

  • Amine-functionalized silicalite nanoparticles increased pDNA transfection efficiency by 230% compared to unfunctionalized ones.
  • Silicalite nanoparticles enhanced PEI-pDNA induced transfection by over 150% in HEK-293 cells.
  • The nanoparticles were found in acidic vesicles or cytoplasm, not the nucleus, and exhibited no significant toxicity at tested concentrations.

Conclusions:

  • Silicalite nanoparticles, particularly when amine-functionalized, represent a promising strategy to enhance non-viral gene delivery efficiency.
  • The sedimentation mechanism and cellular uptake pathways contribute to the observed transfection enhancement.
  • These nanoparticles offer a non-toxic platform for improving gene transfection for potential therapeutic applications.