Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

8.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

2.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.4K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

6.3K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
6.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.1K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.1K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pan-RAS Inhibitors: Expanding Therapeutic Potential and Evading Resistance.

Cancers·2026
Same author

A nanovaccine induces durable and functional MUC4-targeted T cell responses against pancreatic cancer.

Acta biomaterialia·2026
Same author

Peripheral vaccination-induced brain-resident memory CD8+ T cells durably protect mice against intracranial malignancy.

The Journal of clinical investigation·2026
Same author

The Versatile Role of GDF5 in Chondrogenic Progenitor Cell-mediated Cartilage Regeneration via a Hyaluronic Acid-fibrin IPN Hydrogel Platform.

Pharmaceutical research·2026
Same author

Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in a Murine Asthma Model.

Chemical research in toxicology·2026
Same author

ADT-030, a novel PDE10 inhibitor, demonstrates potent antitumor activity in pancreatic ductal adenocarcinoma.

bioRxiv : the preprint server for biology·2026
Same journal

Targeting amino acid metabolism in hepatocellular carcinoma.

Trends in molecular medicine·2026
Same journal

Turning perfusion into repair through ferroptosis blockade.

Trends in molecular medicine·2026
Same journal

CaMKK2: A tumor stress-integration node.

Trends in molecular medicine·2026
Same journal

Precision gene editing: From proof-of-concept to curative therapies.

Trends in molecular medicine·2026
Same journal

Targeting ferroptosis halts graft deterioration in transplantation.

Trends in molecular medicine·2026
Same journal

Amyotrophic lateral sclerosis: A rare but aggressive adult-onset disease.

Trends in molecular medicine·2026
See all related articles

Related Experiment Video

Updated: May 7, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

9.8K

Targeting the NAD+-PARP1-XRCC1 axis in ALS.

Md Ibrahim1, Md Shahadat Hossain1, Lezanne Ooi2

  • 1Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.

Trends in Molecular Medicine
|May 5, 2026
PubMed
Summary
This summary is machine-generated.

Amyotrophic lateral sclerosis (ALS) involves DNA damage and NAD+ depletion, impacting motor neurons. Targeting the NAD+-PARP1-XRCC1 pathway offers a potential therapeutic strategy for ALS treatment.

Keywords:
DNA damage repairNAD(+) metabolismPARP1 hyperactivationXRCC1 dysfunctionamyotrophic lateral sclerosisneuroprotection

More Related Videos

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

3.5K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

5.0K

Related Experiment Videos

Last Updated: May 7, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

9.8K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

3.5K
Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications
10:12

Author Spotlight: Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

5.0K

Area of Science:

  • Neuroscience
  • Genetics
  • Biochemistry

Background:

  • Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease with limited treatment options.
  • Oxidative DNA damage, impaired DNA repair, and NAD+ depletion contribute to motor neuron loss in ALS.
  • The NAD+-PARP1-XRCC1 pathway links DNA repair to cellular energy metabolism.

Purpose of the Study:

  • To review mechanistic and translational evidence for targeting the NAD+-PARP1-XRCC1 axis in ALS.
  • To propose a therapeutic strategy for ALS based on this pathway.

Main Methods:

  • Review of existing mechanistic and translational research on the NAD+-PARP1-XRCC1 axis in ALS.
  • Integration of evidence to support therapeutic targeting.

Main Results:

  • Dysregulation of the NAD+-PARP1-XRCC1 axis can lead to persistent PARP1 activation, failed DNA repair, and energy collapse.
  • This pathway is a viable therapeutic target for ALS.

Conclusions:

  • A staged translational approach is proposed, starting with repurposing PARP1 inhibitors and NAD+ support.
  • Future steps include CNS-directed delivery of repair factors, using biomarkers to monitor treatment efficacy.