Fabrication of mediator-free glutamate sensors based on glutamate oxidase using smart micro-devices

L-glutamate sensor based on glutamate oxidase (GLOx) has been fabricated on N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) activated thioglycolic acid (TGA) self-assembled monolayer (SAM) on smart bio-device using mediator-free simple phosphate buffer system (PBS), where TGA-SAM is used as an enzyme immobilization background. The glutamate sensor displayed a low detection limit (DL, 0.089 +/- 0.002 microM), a wide linear dynamic range (LDR, 0.1 microM to10.0 mM), good linearity (R = 0.9931), and higher sensitivity (20.75 +/- 1.0/microM)(-1)cm-2), small sample volume (70.0 microl), as well as good stability and reproducibility. The smart-biodevice system exemplified a simple and efficient approach to the assimilation of GLOx and sensing surface, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications in health care fields.