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Updated: May 30, 2026

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
Bone formation induced by growth factors embedded into the nanostructured particles
S Facca1, A Ferrand, C Mendoza-Palomares
1Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 977 Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France.
Abstract:
Tissue engineering has merged with stem cell biotechnology with development of new sources of transplantable biomaterials for the treatment of bone tissue diseases. Bone defects are expected to benefit from this new biotechnology because of the low self-regenerating capacity of bone matrix secreting cells. The differentiation of stem cells to bone cells using bi-functionalized multilayered particles is presented. The functionalized particles are composed of poly-glutamic acid (PGA) and poly-L-lysine (PLL) with two bone growth factors (BMP-2 and TGFbeta1) embedded into the multilayered film. The induction of bone from these bioactive particles incubated with embryonic stem cells was demonstrated in vitro. We report the demonstration of a multilayered particle-based delivery system for inducing bone formation in vivo. This new strategy is an alternative approach for in vivo bone formation.
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