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ras induced lesions in a heterotopic mouse bladder.
H E Wagner1, A D Joyce, K Beatrice
1New England Deaconess Hospital, Laboratory of Cancer Biology, Boston, Massachusetts 02115.
Oncogene
|April 1, 1990
Summary
Introducing the HaSV ras transforming gene into normal urothelium induced hyperplastic lesions in vivo. These changes mimic preneoplastic alterations observed in bladder carcinogenesis, highlighting the role of H-ras oncogenic protein.
Area of Science:
- Oncology
- Urology
- Molecular Biology
Background:
- Ras oncogenes are implicated in various cancers.
- Understanding the in vivo effects of ras oncogene expression in bladder epithelium is crucial for carcinogenesis research.
Purpose of the Study:
- To determine the in vivo phenotype of bladder epithelium after HaSV ras transforming gene expression.
- To investigate the role of H-ras oncogenic protein in urothelial hyperplasia and preneoplastic changes.
Main Methods:
- Normal adult bladder mucosa was incubated with HaSV, with or without helper virus.
- Transplanted modified urothelium under the renal capsule of syngeneic animals.
- Histological evaluation and antibody markers (D66, H10) were used to assess cellular changes.
Main Results:
- HaSV exposure increased proliferative potential in urothelial and submucosal elements.
- Hyperplastic lesions (mild-severe) were observed, characterized by increased basal cells and loss of differentiated superficial cells.
- Severe hyperplasia showed disorganized basement membrane, irregular laminin staining, and increased vasculature.
Conclusions:
- The H-ras oncogenic protein induces hyperplastic lesions in normal urothelium.
- These lesions resemble preneoplastic changes in bladder carcinogenesis.
- Ras oncogene expression significantly alters urothelial phenotype in vivo.