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Updated: May 30, 2026

Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Respiratory and TCA cycle activities affect S. cerevisiae lifespan, response to caloric restriction and mtDNA
Erich B Tahara1, Kizzy Cezário, Nadja C Souza-Pinto
1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Cidade Universitária, Av. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil 05508-000.
Abstract:
We studied the importance of respiratory fitness in S. cerevisiae lifespan, response to caloric restriction (CR) and mtDNA stability. Mutants harboring mtDNA instability and electron transport defects do not respond to CR, while tricarboxylic acid cycle mutants presented extended lifespans due to CR. Interestingly, mtDNA is unstable in cells lacking dihydrolipoyl dehydrogenase under CR conditions, and cells lacking aconitase under standard conditions (both enzymes are components of the TCA and mitochondrial nucleoid). Altogether, our data indicate that respiratory integrity is required for lifespan extension by CR and that mtDNA stability is regulated by nucleoid proteins in a glucose-sensitive manner.
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