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Updated: May 30, 2026

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds
Published on: October 15, 2019
Coumarin-caged dG for improved wavelength-selective uncaging of DNA
Clara Menge1, Alexander Heckel
1Frankfurt Institute for Molecular Life Sciences (FMLS), Goethe University of Frankfurt, Max-von-Laue-Str. 15, 60438 Frankfurt (M.), Germany.
Abstract:
Herein we report on diethylaminocoumarin (DEACM) as a new photoremovable protecting group for 2'-deoxyguanosine in oligonucleotides. An oligonucleotide with O(6)-DEACM-caged dG was synthesized and photochemically analyzed. The DEACM group shows superior photochemical properties at 405 nm with an uncaging efficiency (ε·φ) for deprotection that is 17 times higher than that for 2-(o-nitrophenyl)-propyl NPP caging groups in the same position. Wavelength-selective deprotection in the presence of NPP groups proceeds up to 80 times faster.
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