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Molecular identification of a causative parasite species using formalin-fixed paraffin embedded (FFPE) tissues of a
Supinda Koonmee1, Pewpan M Intapan, Hiroshi Yamasaki
1Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Abstract:
PCR-based molecular diagnosis was made for the identification of causative agents of the clinically suspected pulmonary proliferative sparganosis case found in Thailand using formalin-fixed paraffin-embedded (FFPE) biopsy specimens. As a reference, FFPE biopsy specimen from a typical cutaneous sparganosis case was examined together. DNA samples were extracted from tissues and two partial fragments of cytochrome c oxidase subunit 1 (cox1) gene were amplified for the detection of Spirometra DNA. Two cox1 fragments were amplified successfully for both specimens. After alignment of nucleotide sequences of the PCR-amplicons, the causative agents of both cases were identified as Spirometra erinaceieuropaei.
