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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 30, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Phosphoproteomics using iTRAQ.

Alexandra M E Jones1, Thomas S Nühse

  • 1The Sainsbury Laboratory, Norwich Research Park, Norwich, UK. alex.jones@sainsbury-laboratory.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|August 13, 2011
PubMed
Summary
This summary is machine-generated.

Quantitative analysis of phosphopeptides is crucial for understanding cell signaling. This study explores using isobaric tags for relative and absolute quantitation (iTRAQ) to analyze these important protein modifications.

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

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Last Updated: May 30, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Protein phosphorylation is a key regulator of cellular processes, including intracellular signaling.
  • Advances in mass spectrometry (MS) and enrichment techniques have enabled widespread identification of protein phosphorylation.
  • Quantitative analysis of phosphopeptides is essential due to the dynamic nature of this modification.

Purpose of the Study:

  • To demonstrate the utility of isobaric tags for relative and absolute quantitation (iTRAQ) in phosphopeptide analysis.
  • To showcase applications of iTRAQ for quantitative phosphoproteomics across diverse sample types.

Main Methods:

  • Utilized isobaric tags for relative and absolute quantitation (iTRAQ) technology.
  • Applied iTRAQ to the analysis of phosphopeptides.
  • Investigated various biological sample materials.

Main Results:

  • Successfully applied iTRAQ for the quantitative analysis of phosphopeptides.
  • Demonstrated the versatility of iTRAQ across different sample matrices.
  • Provided quantitative data on phosphopeptide abundance.

Conclusions:

  • Isobaric tags for relative and absolute quantitation (iTRAQ) are effective tools for quantitative phosphoproteomics.
  • iTRAQ facilitates the study of dynamic phosphorylation events in biological systems.
  • This approach aids in understanding signaling pathways regulated by protein phosphorylation.