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[HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats]
Lizhi Yan1, Yingfeng Wang, Qirong Yu
1Pony Testing Technology Co., Ltd., Beijing 100080, China. yanlizhi601@163.com
Objective:
To analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.
Method:
Rat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.
Result:
In rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.
Conclusion:
The methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.
