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Two-photon Lysotrackers for in vivo imaging
Ji Hoon Son1, Chang Su Lim, Ji Hee Han
1Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
The Journal of Organic Chemistry
|August 16, 2011
Summary
New two-photon Lysotrackers (CLT-blue and CLT-yellow) enable long-term visualization of lysosomes in live cells and tissues using two-photon microscopy. These probes facilitate colocalization studies with other biological targets.
Area of Science:
- Cell Biology
- Microscopy
- Biochemistry
Background:
- Lysosomes are crucial organelles involved in cellular degradation.
- Visualizing lysosomes in live cells and tissues is essential for understanding cellular processes.
- Existing imaging techniques may have limitations in long-term live-cell lysosome visualization.
Purpose of the Study:
- To develop novel two-photon fluorescent probes for lysosome imaging.
- To enable long-term, high-resolution visualization of lysosomes in live biological samples.
- To facilitate colocalization studies with other cellular targets using two-photon microscopy.
Main Methods:
- Synthesis and characterization of two-photon Lysotrackers (CLT-blue and CLT-yellow).
- Excitation with 750-840 nm femtosecond laser pulses and emission detection at 470 nm (CLT-blue) and 550 nm (CLT-yellow).
- Loading probes into live cells and tissue slices for two-photon microscopy imaging.
Main Results:
- CLT-blue and CLT-yellow probes exhibit efficient two-photon excitation and emission.
- Probes allow for stable, long-term visualization of lysosomes in live cells and tissues.
- Demonstrated successful loading and imaging in cellular and tissue environments.
Conclusions:
- Two-photon Lysotrackers (CLT-blue and CLT-yellow) are effective tools for lysosome imaging.
- These probes significantly enhance the capabilities of two-photon microscopy for live-cell and tissue studies.
- The developed probes are valuable for colocalization experiments and advancing lysosome research.
