Detection of bacterial diversity in rat's periodontitis model under imitational altitude hypoxia environment
Xian Xiao1, Yan Li, Gang Zhang
1The First Outpatient Department of Chengdu Military District Institution, Chengdu Army General Hospital, Chengdu, PR China.
Objectives:
Oral epidemiologic investigations in China western territory have showed that the immigrants in the plateau have a higher morbidity with periodontitis. To find the possible relationship between the pathogenesis of periodontitis and altitude hypoxia, we designed a periodontitis rat model via housed in low pressure oxygen chamber and investigated the bacterial diversity in the gingival crevicular fluid (GCF).
Design:
Eighty Sprague-Dawley rats were divided into CON-normal, CON-hypoxia, EXP-normal and EXP-hypoxia group, without or with periodontal induce, breeding in normal environment or altitude hypoxia environment. Periodontal parameters (including gingival index, GI, and tooth mobility, TM) were measured after 2, 4, 6 and 8 weeks; periodontal samples were collected for histological analysis after rats were sacrificed at the 8th week. The 16S rDNA of bacteria in GCF was amplified by PCR at the 8th week, and separated by the denaturing gradient gel electrophoresis (DGGE) approach.
Results:
EXP-hypoxia group's GI and TM showed later and more severe periodontal tissue damage than EXP-normal (p<0.05 or 0.01). The histologic analyses did not find any pathologic difference between EXP-hypoxia and EXP-normal groups except for a slight difference on the lesion degree. By the DGGE analyses, the bacteria of five samples in the same group showed high concordance, but the bacteria in the different groups showed a great diversity.
Conclusion:
The course of periodontitis in altitude hypoxia environment is later than normal, but the degree of periodontal lesion was more severe and microbial community in GCF can be affected by the altitude hypoxia environment.
