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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Trait stacking in transgenic crops: challenges and opportunities.

Qiudeng Que1, Mary-Dell M Chilton, Cheryl M de Fontes

  • 1Syngenta Biotechnology, Inc., Research Triangle Park, NC, USA. qiudeng.que@syngenta.com

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Transgenic crops with stacked traits are increasing, developed through conventional breeding and molecular stacking. This review covers current products, technologies, and challenges in assembling and expressing multiple traits in crops.

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Area of Science:

  • Agricultural Biotechnology
  • Plant Genetics
  • Molecular Biology

Background:

  • The cultivation of transgenic crops with stacked traits has significantly increased.
  • Stacked traits are often developed through conventional breeding by crossing single-trait events.
  • Companies are developing products with multiple insect and herbicide tolerance genes.

Purpose of the Study:

  • To review the current status of stacked trait products in crops.
  • To examine the technologies used for trait stacking, including conventional breeding and molecular methods.
  • To identify and discuss the technical challenges associated with stacked trait development and expression.

Main Methods:

  • Review of existing literature on transgenic crop development and trait stacking.
  • Analysis of current industry trends in stacked trait product development.
  • Examination of molecular techniques for assembling multiple transgenes.

Main Results:

  • Conventional breeding is the primary method for creating current stacked trait products.
  • Technologies for molecular stacking of multiple traits into a single locus are advancing.
  • Challenges exist in assembling large transgene arrays, their delivery, and ensuring transgene expression.

Conclusions:

  • Stacked trait technology is rapidly evolving, driven by the need for broad pest and weed control.
  • Molecular stacking offers potential for more efficient trait integration.
  • Further research is needed to overcome challenges in transgene assembly, delivery, and expression for advanced stacked trait crops.