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Updated: May 30, 2026

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
Chitosan interaction with iron from yoghurt using an in vitro digestive model: comparative study with plant dietary
Marina Dello Staffolo1, Miriam Martino, Alicia Bevilacqua
1Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), CONICET-CCT La Plata, Fac. Cs. Exactas, Universidad Nacional de La Plata, La Plata, 47 y 116, 1900, Argentina; E-Mails: mmartino@ing.unlp.edu.ar (M.M.); aebevila@ing.unlp.edu.ar (A.B.).
Abstract:
The objective of this work was to investigate the interaction of chitosan with iron from yoghurt by an in vitro gastrointestinal tract model. Taking into account that chitosan is a polysaccharide included in fiber definition by Codex Alimentarius; chitosan behavior was studied and compared with different plant fiber (wheat, bamboo, apple, psyllium and inulin) behaviors, in the same in vitro conditions. Ferrous sulfate was added to yoghurts with each type of fiber. The gastric environment was simulated with HCl (pH 1.0-2.0). The duodenal environment was simulated with NaHCO(3) (pH 6.8-7.2) and a dialysis tubing cellulose membrane. Results showed that chitosan had the highest iron retention percentages (53.2% at 30 min; 56.8% at 60 min) interacting in a more pronounced manner with iron than the plant fibers used in this work.
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