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Updated: May 30, 2026
2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia
Published on: June 22, 2013
[Expression and function of autophagy after ischemia/reperfusion in rats hippocampus neuron]
Fang Su1, Pei Zhang, Zhi-Wei Jiang
1Department of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
Objective:
To explore the expression of autophagy after ischemia/reperfusion and its possible function in rats hippocampus neurons.
Methods:
After 2 hours oxygen-glucose deprivation and different periods time of reperfusion (OGD/R) treatment in primary hippocampal neurons, neuron viability was evaluated by MTT assay, specific structure of autophagosome and specific protein of autophagy microtubule-associated protein 1 light chain 3 B (LC3B) were detected by transmission electron microscope and immunofluorescence respectively. The inhibitor of autophagy 3-Methyladenine (3-MA) was also used to exam the viability of neurons.
Results:
Treatment by OGD/R markedly reduced neuronal viability. Compared to the control group, autophagy existed in different time periods after OGD/R shown both in transmission electron microscope and immunofluorescence. Application of 3-MA significantly reduced neuronal viability.
Conclusion:
Oxygen-glucose deprivation can activate autophagy in rat hippocampus neurons, which may resist the injury during ischemia/reperfusion.
