Related Experiment Video
Updated: May 30, 2026
06:08
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
Obesity short-circuits stemness gene network in human adipose multipotent stem cells
Mar Roldan1, Manuel Macias-Gonzalez, Regina Garcia
1Fundacion Instituto Mediterráneo para el Avance de la Biotecnología y la Investigación Sanitaria (IMABIS), Malaga, Spain.
Summary
Obesity impairs the stemness of human omental multipotent stem cells (hO-MSCs). These stem cells show reduced proliferation, increased senescence, and loss of differentiation capacity, indicating a disrupted stemness gene network in obese individuals.
Area of Science:
- Stem cell biology
- Obesity research
- Adipose tissue
Background:
- Adipose multipotent stem cells (hO-MSCs) are crucial for adipose tissue function.
- The influence of the adipogenic environment on stemness remains incompletely understood.
Purpose of the Study:
- To investigate how the adipogenic environment in obesity affects the stemness of hO-MSCs.
- To characterize changes in hO-MSC function and gene regulation in lean versus obese individuals.
Main Methods:
- Isolation, expansion, and characterization of hO-MSCs from healthy lean and morbidly obese individuals.
- Assessment of cell proliferation, senescence, cytokine secretion, and multilineage differentiation capacity.
- Analysis of Wnt, Notch, and Sonic Hedgehog signaling pathways and microRNA expression.
Main Results:
- Obesity correlated with decreased hO-MSC proliferation and premature senescence.
- Increased cytokine secretion and loss of multilineage differentiation capacity were observed in obese individuals.
- Dysregulation of key signaling pathways (Wnt, Notch, Sonic Hedgehog) and upregulation of specific microRNAs were noted.
Conclusions:
- Obesity significantly compromises the stemness of hO-MSCs.
- Obesity disrupts the stemness gene network in hO-MSCs, affecting their proliferation, differentiation, and senescence.
