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Updated: May 30, 2026

10:27
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
Published on: November 10, 2015
Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance
Hye Ran Koh1, Kyu Young Han, Jiwon Jung
1Department of Chemistry, Seoul National University, Seoul 151-747, Korea.
Abstract:
We developed a new single nucleotide polymorphism (SNP) genotyping method based on single-molecule multi-color fluorescence resonance energy transfer (FRET). We demonstrated that this new method uses less than 1 fmol of sample and is also highly quantitative with a detection level of 1% or lower in the minor allele fraction.

