A Unique BSL-3 Cryo-Electron Microscopy Laboratory at UTMB

Area of Science:

• Structural Biology
• Virology
• Microscopy

Background:

• Studying viruses at the molecular level requires advanced imaging techniques.
• High-containment laboratories are essential for research involving dangerous pathogens.
• Cryo-electron microscopy (CryoEM) provides high-resolution 3D structural information.

Purpose of the Study:

• To describe the establishment and operation of a unique cryo-electron microscopy (CryoEM) facility.
• To detail the safety protocols and regulatory approvals for a Biological Safety Level 3 (BSL-3) laboratory dedicated to virus imaging.
• To highlight the facility's capability for select agent research.

Main Methods:

• Implementation of standard operating procedures for safe and efficient laboratory function.
• Utilizing cryo-electron microscopy for high-resolution imaging of viral structures.
• Adherence to strict safety guidelines within a BSL-3 containment environment.

Main Results:

• The W. M. Keck Center for Virus Imaging has operated successfully for over a year.
• The facility received approval from the Centers for Disease Control and Prevention (CDC) for select agent studies.
• The center is the sole BSL-3 CryoEM facility authorized for select agent research.

Conclusions:

• A dedicated BSL-3 CryoEM facility can be safely and effectively operated for studying complex viral structures.
• The established protocols ensure the secure handling of hazardous biological materials.
• This unique facility advances the understanding of virus organization and facilitates critical research.