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Chemical fingerprinting of Equisetum arvense L. using HPTLC densitometry and HPLC
Francesca R Gallo1, Giuseppina Multari, Elena Federici
1Dipartimento del Farmaco, Istituto Superiore di Sanità, V.le Regina Elena 299, Rome I-00161, Italy. francesca.gallo@iss.it
Abstract:
Equisetum arvense L. is a herbaceous medicinal plant, commonly known as horsetail, whose extracts have been reported to possess diuretic and haemostatic properties. The aim of this study was to evaluate the use of fingerprint chromatographic methods on commercially available raw materials or preparations of E. arvense L. in order to ascertain their quality and identify possible adulterants using HPLC and HPTLC densitometry. Two chromatographic methods were used to determine the chemical fingerprints of E. arvense and other allied species. The first was based on HPTLC identification followed by densitometric measurement at 350 nm. The second was based on HPLC separation. The ease of sample preparation and the possibility of simultaneous analysis of several samples in a short time make HPTLC a method of choice for the comprehensive quality evaluation of herbal products.
