Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging
Cledir Santos1, Nelson Lima, Paula Sampaio
1IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal.
Abstract:
Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensis, and C. albicans and C. dubliniensis could be clearly separated. MALDI-TOF-ICMS stands out as a promising tool for the rapid detection of emerging pathogens.
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