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Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 30, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

DNA-templated fluorescent silver nanoclusters.

Bingyan Han¹, Erkang Wang

¹State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin, China.

Analytical and Bioanalytical Chemistry

|August 23, 2011

Summary

This summary is machine-generated.

DNA-templated fluorescent silver nanoclusters offer excellent properties for various applications. Their synthesis and uses, including bioimaging and biosensing, are reviewed here.

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Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Fluorescent silver nanoclusters (AgNCs) are emerging nanomaterials with unique optical properties.
DNA serves as a versatile template for synthesizing AgNCs with controlled size and fluorescence.
Aqueous synthesis offers an environmentally friendly and biocompatible approach.

Purpose of the Study:

To review the synthesis strategies for DNA-templated fluorescent silver nanoclusters.
To explore the diverse applications of these nanoclusters in aqueous solutions.
To highlight the advantages of using DNA as a template for AgNCs.

Main Methods:

Oligonucleotide sequences and conformations are employed to direct AgNC formation.
Characterization techniques confirm the size, structure, and fluorescence of AgNCs.
Various assay formats are utilized to demonstrate application performance.

Main Results:

Diverse DNA sequences and structures yield AgNCs with tunable fluorescence.
Applications demonstrated include live cell imaging, metal ion sensing, and small biomolecule detection.
Detection of DNA and proteins using AgNCs has also been achieved.

Conclusions:

DNA-templated synthesis provides a robust method for generating highly fluorescent AgNCs.
These nanoclusters show significant potential in various bioanalytical and imaging applications.
Further development promises expanded utility in diagnostics and sensing.