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Updated: May 30, 2026
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Published on: April 16, 2013
A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on
António F Sousa1, Pedro Z Andrade, Roksana M Pirzgalska
1Department of Bioengineering, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Technical University of Lisbon, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
Abstract:
A novel cell separation process based on immunoaffinity aqueous two phase systems is presented to isolate and purify CD34(+) stem/progenitor cells directly from the whole umbilical cord blood (UCB). A system, composed of polyethylene glycol and dextran, was evaluated for the selective recovery of CD34(+) cells from UCB. A monoclonal antibody against the CD34 surface antigen was used for the direct partitioning of CD34(+) cells in UCB to the PEG-rich phase. The initial population of CD34(+) cells (0.2% of the initial sample) was enriched to values up to 42% in a single partitioning step, while the majority of contaminant cells were partitioned to the dextran-rich phase (1.37 × 10(-2) < K(P) < 2.76 × 10(-2)). This novel selection method allowed a recovery yield of 95% of CD34(+) cells with a purification factor of 245 and is expected to pave a new way to purify hematopoietic stem/progenitor cells for use in a variety of clinical settings.
