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In vivo analyses of integrin beta 1 subunit function in fibronectin matrix assembly
T Darribère1, K Guida, H Larjava
1Université Pierre et Marie Curie, Unité Associèe Centre National de la Recherche Scientifique, Paris, France.
The Journal of Cell Biology
|May 1, 1990
Summary
Fibronectin fibrillogenesis in Pleurodeles waltl development relies on cell surface integrins and the RGD signal. Blocking these essential components reversibly inhibits fibronectin assembly and delays embryonic development.
Area of Science:
- Developmental Biology
- Cell Biology
- Extracellular Matrix Research
Background:
- Early amphibian development involves fibronectin (FN) fibrillogenesis, forming a complex extracellular matrix.
- Fibronectin assembly into fibrils begins at the early blastula stage on the blastocoele roof.
Purpose of the Study:
- To investigate the in vivo mechanisms regulating fibronectin fibril formation during early urodele amphibian development.
- To determine the roles of cell surface receptors and specific recognition sequences in fibronectin assembly.
Main Methods:
- In vivo analysis using labeled fibronectin (FN) and synthetic peptides with the Arg-Gly-Asp (RGD) sequence.
- Utilized antibodies against the integrin beta 1 subunit and its cytoplasmic domain.
- Investigated effects of exogenous FN, RGD peptides, and anti-integrin antibodies on fibrillogenesis.
Main Results:
- Exogenous mammalian FN assembled into fibrils in amphibian embryos, mirroring endogenous FN patterns, indicating cell surface regulation.
- Fibrillogenesis was dose-dependently inhibited by RGD peptides and antibodies to the integrin beta 1 subunit.
- Intracellular antibody injection targeting the integrin beta 1 cytoplasmic domain caused reversible inhibition of FN fibril formation, affecting cell lineages and development.
Conclusions:
- Integrin beta 1 subunit and the RGD recognition signal are crucial for proper fibronectin fibril assembly in early amphibian development.
- Cell surface interactions, mediated by integrins, are key regulators of fibronectin extracellular matrix formation.
- Disruption of these pathways leads to developmental delays, highlighting the importance of fibronectin assembly.