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Updated: May 30, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
Published on: May 15, 2012
A new strategy for the synthesis of bisaminoacylated tRNAs
Ryan C Nangreave1, Larisa M Dedkova, Shengxi Chen
1Center for BioEnergetics, The Biodesign Institute, Arizona State University, Tempe, Arizona 85827, USA.
Abstract:
Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C(OH)), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.
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