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Immunogold Electron Microscopy

Immunogold Electron Microscopy

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

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Published on: November 13, 2021

New method for affinity adsorbent screening using gold colloids.

Jun Wang¹, Wei Wang, Qinqin Wei

¹Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University, Tianjin 300071, PR China.

Langmuir : the ACS Journal of Surfaces and Colloids

|August 25, 2011

Summary

This summary is machine-generated.

This study introduces a novel gold colloid method for screening adsorbent ligands. Dpa-Zn(2+) demonstrated the highest affinity, enabling efficient selection of effective adsorbents for phosphorylated peptides.

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Last Updated: May 30, 2026

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Area of Science:

Materials Science
Analytical Chemistry
Biotechnology

Background:

Screening adsorbent ligands is crucial for developing efficient separation and purification technologies.
Traditional methods can be time-consuming and require significant sample volumes.
Developing rapid, sensitive, and cost-effective screening methods is essential.

Purpose of the Study:

To develop a rapid and effective method for screening adsorbent ligands using gold colloid optical properties.
To identify high-affinity ligands for the phosphorylated peptide CGGFGGpSG.
To validate screening results with quantitative adsorption experiments.

Main Methods:

Modification of gold nanoparticles (AuNPs) with a phosphorylated peptide (CG8-AuNPs).
Copolymerization of candidate ligands (Dpa-Zn(2+), DMAPAA, AAn) with acrylamide to form polymers.
Induction of CG8-AuNPs aggregation by candidate ligands and monitoring color changes.
Statistical adsorption experiments to determine adsorption capacity.

Main Results:

Dpa-Zn(2+) exhibited the highest ligand affinity, inducing a color change at 10 μM.
DMAPAA showed affinity at 40 μM, while AAn showed minimal interaction.
The adsorbent A-AAm-Dpa-Zn(2+) demonstrated the highest adsorption capacity (426 mg/g) for CG-8.
The developed method requires low sample consumption and can be visually monitored.

Conclusions:

The gold colloid-based optical screening method is rapid, sensitive, and effective for evaluating ligand-adsorbate affinity.
Dpa-Zn(2+) is a highly effective ligand for the phosphorylated peptide CGGFGGpSG.
This technique offers a simple, visual, and efficient approach for adsorbent screening in various applications.