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Updated: May 30, 2026
11:42
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Intrastrand locks increase duplex stability and base pairing selectivity
Cora Prestinari1, Clemens Richert
1Institute for Organic Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70565 Stuttgart, Germany.
Chemical Communications (Cambridge, England)
|August 25, 2011
Abstract:
Oligodeoxynucleotide probes with disulfide locks between neighboring nucleobases show increases in melting point for duplexes with RNA target strands of up to 7.6 °C. The weakly pairing TT dimers are replaced with locked 2'-deoxy-5-(thioalkynyl)uridine residues via automated synthesis.
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