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Related Concept Videos

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Receptor-Mediated Endocytosis01:20

Receptor-Mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

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Related Experiment Video

Updated: May 29, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

Visualizing receptor endocytosis and trafficking.

Ali Salahpour1, Larry S Barak

  • 1Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2011
PubMed
Summary
This summary is machine-generated.

This chapter details methods for studying G-protein-coupled receptors (GPCRs) internalization and trafficking. Understanding these processes is key for drug development targeting these crucial membrane proteins.

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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
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Related Experiment Videos

Last Updated: May 29, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
12:40

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Published on: October 20, 2014

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07:48

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Published on: July 3, 2015

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) are the largest class of membrane protein drug targets.
  • GPCR-7TMRs mediate cellular responses to diverse ligands, including hormones and small molecules.
  • Receptor desensitization, internalization, and resensitization are critical regulatory steps following GPCR activation.

Purpose of the Study:

  • To describe technical approaches for studying GPCR internalization.
  • To provide insights into GPCR trafficking mechanisms.
  • To aid researchers in investigating GPCR-ligand interactions and signaling regulation.

Main Methods:

  • Describes various technical approaches for studying GPCR internalization.
  • Focuses on methodologies for analyzing receptor trafficking dynamics.
  • Highlights techniques relevant to understanding GPCR regulation.

Main Results:

  • Provides a comprehensive overview of methods for GPCR internalization studies.
  • Illustrates the importance of receptor trafficking in signal regulation.
  • Offers practical guidance for experimental design in GPCR research.

Conclusions:

  • Technical approaches for studying GPCR internalization and trafficking are essential for understanding receptor function.
  • The described methods facilitate research into GPCR-mediated signaling and drug development.
  • Investigating GPCR trafficking is crucial for optimizing therapeutic strategies targeting these receptors.