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Updated: May 29, 2026
Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Establishment and characterization of an immortalized human dermal papilla cell line
Seung Hyun Shin1, Sang Yoon Park, Moon Kyu Kim
1Department of Immunology and Hair Research Center, School of Medicine, Kyungpook National University, Daegu, Korea.
Abstract:
Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40TDPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/β-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.
