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Cloning and identification of microRNAs in earthworm (Eisenia fetida)

Xue-Mei Huang1, Qing-Nan Tian, Zhen-Xia Bao

  • 1Department of Bioengineering, Zhengzhou University, No. 100 of Science Road, Zhengzhou, Henan, 450001, China.

Biochemical Genetics
|August 30, 2011
PubMed

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MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...