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Updated: Jul 20, 2026

Efficient Sporulation of Saccharomyces cerevisiae in a 96 Multiwell Format
Published on: September 17, 2016
Transcriptional control of SSL1, a gene controlling alpha-specific inactivation of a-factor in Saccharomyces
Abstract:
The SSL1 gene of S. cerevisiae which is involved in the cell-type specific recovery of mating type alpha cells from cell cycle arrest by the mating hormone a-factor has been cloned as a 1 kb Sau3A fragment in a complementing plasmid from a library of S. cerevisae genomic DNA. Disruption of this gene in wild-type alpha-cells results in the ssl1- phenotype which is characterized by an extreme sensitivity to a-factor. Transcription analysis revealed that the SSL1 gene is transcribed only in MAT alpha cells but not in MAT alpha or diploid (MAT alpha/MAT alpha) cells defining it as a member of the set of alpha-specific genes.
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