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Updated: Jul 20, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
Published on: August 26, 2012
The carboxyl-terminal repeat domain of RNA polymerase II is not required for transcription factor Sp1 to function in
1Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Abstract:
We show that the mammalian transcription Sp1 stimulates accurate transcription in a partially fractionated RNA polymerase II-dependent system from Drosophila cultured cells. Moreover, the extent of stimulation is equal for intact RNA polymerase II (polymerase IIA) and polymerase lacking the unique carboxyl-terminal domain of the largest subunit (polymerase IIB). We conclude that in this system Sp1 interacts with a component of the transcription machinery, other than the carboxyl-terminal domain, which is preserved between mammals and insects.
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