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Updated: May 29, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Lassa virus nucleoprotein mutants generated by reverse genetics induce a robust type I interferon response in human
Xavier Carnec1, Sylvain Baize, Stéphanie Reynard
1Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris cedex 15, France.
Abstract:
Lassa virus (LASV; Arenaviridae) is responsible for severe hemorrhagic fevers in Africa. LASV nucleoprotein (NP) plays important roles in regulating viral transcription and replication and in inhibiting type I interferon (IFN) production. The NP C-terminal domain contains a 3'-to-5' exonuclease activity involved in suppressing IFN induction. We have established a murine polymerase (Pol) I reverse genetics system for LASV, showing that residues D389 and G392 of NP were critical for LASV viability, while the D389A/G392A and D389T/392A double mutants were severely altered in the ability to suppress IFN in macrophages and dendritic cells. Assessing their attenuation in vivo may open new perspectives in vaccinology.
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