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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...

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A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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Published on: May 27, 2014

Mass spectrometry-based quantitative analysis and biomarker discovery.

Naoto Suzuki1

  • 1Laboratory of Oncology, Pharmacy Practice and Sciences, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. nasuzuki@m.tohoku.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|September 2, 2011
PubMed
Summary

Mass spectrometry enables precise analysis of metabolites like arachidonic acid derivatives for disease diagnosis and therapeutic drug monitoring. This approach aids in identifying biomarkers for conditions such as atopic dermatitis and rheumatoid arthritis.

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Area of Science:

  • Clinical chemistry and analytical chemistry
  • Biomarker discovery and metabolomics
  • Medical pharmacy and therapeutic drug monitoring

Background:

  • Mass spectrometry is a key platform for quantitative analysis and biomarker discovery in clinical settings.
  • Metabolites of arachidonic acid, including cysteinyl leukotriene (cysLT), thromboxane (TX), and 12-hydroxyeicosatetraenoic acid (12-HETE), are implicated in various diseases.
  • Therapeutic drug monitoring (TDM) is crucial for managing immunosuppressants like sirolimus.

Purpose of the Study:

  • To review mass spectrometry-based research strategies for analyzing arachidonic acid metabolites and their role in diseases.
  • To present the development and application of sensitive determination methods for endogenous compounds and TDM.
  • To highlight the utility of metabolomics for identifying novel biomarkers in clinical samples.

Main Methods:

  • Development of sensitive determination methods using simple solid-phase extraction for clinical application.
  • Application of mass spectrometry for quantitative analysis of endogenous metabolites and therapeutic drugs.
  • Implementation of non-targeted metabolomics strategies for analyzing biological fluids from cells, animals, and humans.

Main Results:

  • Established relationships between specific arachidonic acid metabolites and diseases like atopic dermatitis, rheumatoid arthritis, and diabetes mellitus.
  • Developed practical quantitative methods for TDM, exemplified by sirolimus monitoring in organ transplant recipients.
  • Successfully applied metabolomics to differentiate groups based on altered metabolite patterns and identify potential biomarkers.

Conclusions:

  • Mass spectrometry-based metabolomics offers a powerful approach for disease diagnosis, prognosis, and treatment assessment.
  • Sensitive analytical methods and metabolomics strategies can provide valuable insights into disease mechanisms and biomarker discovery.
  • This research contributes to the advancement of medical pharmacy through improved analytical techniques and biomarker identification.