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Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
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A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody

G H Lopez1, M M Dean, K Yasui

  • 1Australian Red Cross Blood Service, Brisbane, Qld, Australia.

Vox Sanguinis
|September 3, 2011
PubMed

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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...