Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondria01:37

Mitochondria

13.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.4K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

7.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.6K
Autophagy01:27

Autophagy

5.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.0K
Autophagic Cell Death01:18

Autophagic Cell Death

3.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.3K
Cellular Adaptation I: Introduction and Atrophy01:23

Cellular Adaptation I: Introduction and Atrophy

70
Cells can adapt to environmental changes to maintain function and avoid injury, a process called cellular adaptation. Adapted cells exist in a reversible intermediate state with changes in size, number, phenotype, metabolism, or function. These responses help cells meet altered physiological or pathological demands; for example, enlargement of breast and uterine tissues during pregnancy. Early adaptations may enhance function, but persistent stress eventually causes tissue damage.Types of...
70
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

99
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
99

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of mitochondrial lipid peroxidation in cells and zebrafish using a targeted mass spectrometry probe.

Cell chemical biology·2026
Same author

IRE1 regulates the proteostasis of TDP-43/TARDBP in ALS/FTD through ribosome-associated quality control.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

TRIM21 induces selective autophagy of viruses and bacteria.

Molecular cell·2026
Same author

Where and how do mammalian cells shape autophagosomes?

The Journal of biological chemistry·2026
Same author

Author Correction: p300 nucleocytoplasmic shuttling underlies mTORC1 hyperactivation in Hutchinson-Gilford progeria syndrome.

Nature cell biology·2025
Same author

Monitoring Autophagosome Biogenesis and Degradation in Mammalian Cells Using LC3B Blotting.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: May 3, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

33.9K

Autophagy and aging.

David C Rubinsztein1, Guillermo Mariño, Guido Kroemer

  • 1Department of Medical Genetics, University of Cambridge, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK. dcr1000@hermes.cam.ac.uk

Cell
|September 3, 2011
PubMed
Summary
This summary is machine-generated.

Autophagy, a cellular recycling process, declines with age. Enhancing autophagy may combat aging and promote longevity by reversing age-related tissue damage.

More Related Videos

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.0K
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

5.7K

Related Experiment Videos

Last Updated: May 3, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

33.9K
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.0K
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

5.7K

Area of Science:

  • Cellular Biology
  • Gerontology
  • Molecular Biology

Background:

  • Autophagy, a fundamental cellular degradation process, is implicated in aging.
  • Reduced autophagic activity is a hallmark of both normal and pathological aging.
  • Autophagy dysfunction contributes to age-related degenerative changes in mammalian tissues.

Purpose of the Study:

  • To explore the causal relationship between impaired autophagy and aging.
  • To elucidate the molecular mechanisms underlying autophagy's anti-aging effects.
  • To review how interventions affecting lifespan modulate autophagy.

Main Methods:

  • Review of existing literature on autophagy and aging.
  • Analysis of genetic and pharmacological studies in model organisms.
  • Discussion of molecular pathways linking autophagy to longevity.

Main Results:

  • Genetic inhibition of autophagy mimics aging phenotypes.
  • Lifespan-extending interventions often enhance autophagy.
  • Autophagy inhibition negates the benefits of various longevity interventions.

Conclusions:

  • Perturbed autophagy is strongly linked to aging processes.
  • Modulating autophagy holds potential for anti-aging strategies.
  • Understanding autophagy's role is crucial for developing interventions to promote healthy aging.