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Updated: May 29, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Postsynthetic modification of oligonucleotides with imidazophenazine dye and its effect on duplex stability
Larysa Dubey1, Olga Ryazanova, Victor Zozulya
1Institute of Molecular Biology and Genetics, National Academy of Sciences, Kyiv, Ukraine.
Abstract:
Carboxyalkyl derivative of the intercalating agent imidazo[4,5-b]phenazine was used for the postsynthetic oligonucleotide modification. Model pentadecathymidylate-imidazophenazine conjugate was prepared from 5'-aminoalkyl-modified (dT)(15) by using phosphonium coupling reagent BOP in the presence of 1-hydroxybenzotriazole. Spectral-fluorescent properties of the conjugate were studied. The attachment of the dye was found to increase the thermal stability of (dT)(15) duplex with poly(dA) by more than 4°C, probably by the intercalation mechanism.
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