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Updated: May 29, 2026

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
05:35

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Reprint of: Immobilized-Metal Affinity Chromatography (IMAC): A Review

Helena Block1, Barbara Maertens, Anne Spriestersbach

  • 1QIAGEN GmbH, Qiagen Strasse 1, Hilden, Germany.

Protein Expression and Purification
|September 7, 2011
PubMed

This article reviews the development of immobilized-metal affinity chromatography (IMAC) and describes its most important applications. We provide an overview on the use of IMAC in protein fractionation and proteomics, in protein immobilization and detection, and on some special applications such as purification of immunoglobulins and the Chelex method. The most relevant application- purification of histidine-tagged recombinant proteins-will be reviewed in greater detail with focus of state-of-the-art materials, methods, and protocols, and the limitations of IMAC and recent advances to improve the technology and the methods will be described.

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