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Updated: May 29, 2026
Gold Nanoparticle Synthesis
Published on: July 10, 2021
Improving the yield of mono-DNA-functionalized gold nanoparticles through dual steric hindrance
Zhengtao Li1, Enjun Cheng, Wenxiao Huang
1Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, No. 11 Zhongguancun Beiyitiao, Beijing 100190, PR China.
Abstract:
A novel strategy of dual steric hindrance, which was obtained by Janus modification of gold nanoparticles (Au NPs) and volume exclusion of DNA, was adopted to prepare mono-DNA-modified Au NPs. The yield of mono-DNA-functionalized Au NPs significantly improved from 44 to 70% in the reaction between Au NPs and thiolated DNA. Furthermore, the specificity of mono-DNA-functionalized Au NPs was enhanced from 57 to 95%. The as-prepared Au NPs without postsynthetic treatment showed good controllability in self-assembly fabrication of complex nanostructures.
