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UVC mutagenicity is suppressed in Japanese miso-treated human RSa cells, possibly via GRP78 expression

Xia Jiang1, Qian Ren, Shi-Ping Chen

  • 1Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University.

Bioscience, Biotechnology, and Biochemistry
|September 8, 2011
PubMed

Insights

Miso consumption may protect human cells from DNA damage. This study shows miso increases glucose-regulated protein 78 (GRP78) expression, which suppresses mutations caused by ultraviolet C (UVC) radiation.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The role of miso in modulating cellular mutability is largely unknown.
  • Previous observations indicated a link between increased glucose-regulated protein 78 (GRP78) expression and suppressed mutation in human RSa cells post-ultraviolet C (UVC) irradiation.

Purpose of the Study:

  • To investigate whether miso treatment enhances GRP78 expression.
  • To determine if miso treatment suppresses UVC-induced mutagenicity in human RSa cells.

Main Methods:

  • Human RSa cells were treated with water extracts of miso products and their components.
  • GRP78 expression levels were quantified using RT-PCR and immunoblotting analysis.
  • UVC-induced mutations, specifically ouabain resistance (Oua(R)) and K-ras codon 12-base substitutions, were assessed.

Main Results:

  • Miso treatment led to increased GRP78 expression in RSa cells.
  • A significant decrease in UVC-induced Oua(R) and K-ras codon 12-base substitution mutation frequencies was observed in miso-treated cells.
  • The suppressive effect on mutagenicity was diminished in cells where GRP78 was downregulated using GRP78 siRNA.

Conclusions:

  • Miso effectively suppresses UVC-induced mutagenicity in human RSa cells.
  • This suppression appears to be mediated by miso's ability to increase GRP78 expression.

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