An automation-assisted generic approach for biological sample preparation and LC-MS/MS method validation
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Updated: May 29, 2026
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
Jie Zhang1, Shimin Wei, David W Ayres
1Bioanalytics, Department of Drug Metabolism & Pharmacokinetics, Novartis Institutes for Biomedical Research, East Hanover, NJ 07936, USA. Jie-3.Zhang@novartis.com
Background:
Although it is well known that automation can provide significant improvement in the efficiency of biological sample preparation in quantitative LC-MS/MS analysis, it has not been widely implemented in bioanalytical laboratories throughout the industry. This can be attributed to the lack of a sound strategy and practical procedures in working with robotic liquid-handling systems.
Results:
Several comprehensive automation assisted procedures for biological sample preparation and method validation were developed and qualified using two types of Hamilton Microlab liquid-handling robots. The procedures developed were generic, user-friendly and covered the majority of steps involved in routine sample preparation and method validation.
Conclusion:
Generic automation procedures were established as a practical approach to widely implement automation into the routine bioanalysis of samples in support of drug-development programs.
