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Updated: May 29, 2026
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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
An automation-assisted generic approach for biological sample preparation and LC-MS/MS method validation
Jie Zhang1, Shimin Wei, David W Ayres
1Bioanalytics, Department of Drug Metabolism & Pharmacokinetics, Novartis Institutes for Biomedical Research, East Hanover, NJ 07936, USA. Jie-3.Zhang@novartis.com
Bioanalysis
|September 9, 2011
Summary
Automation significantly improves biological sample preparation efficiency for quantitative LC-MS/MS analysis. Generic, user-friendly procedures were developed to facilitate widespread adoption in bioanalytical laboratories supporting drug development.
Area of Science:
- Bioanalysis
- Analytical Chemistry
- Laboratory Automation
Background:
- Automation offers significant efficiency gains in biological sample preparation for quantitative LC-MS/MS.
- Widespread adoption in bioanalytical labs is limited by a lack of practical strategies and procedures for robotic liquid-handling systems.
Purpose of the Study:
- To develop and qualify comprehensive, user-friendly automation-assisted procedures for biological sample preparation and method validation.
- To establish generic procedures for routine bioanalysis in drug development.
Main Methods:
- Utilized two types of Hamilton Microlab liquid-handling robots.
- Developed and qualified automation-assisted procedures covering routine sample preparation and method validation steps.
Main Results:
- Successfully developed and qualified generic, user-friendly automation procedures.
- Procedures demonstrated applicability to routine sample preparation and method validation.
Conclusions:
- Established generic automation procedures as a practical strategy for broad implementation in bioanalytical laboratories.
- These procedures support the routine bioanalysis of samples in drug development programs.
