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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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Producing protein microarrays from DNA microarrays.

Oda Stoevesandt1, Michael J Taussig, Mingyue He

  • 1Protein Technology Group, Babraham Bioscience Technologies Ltd, Cambridge, UK. oda.stoevesandt@bbsrc.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a cost-effective method for producing protein microarrays using cell-free protein synthesis from a DNA template. This innovation makes protein arraying accessible without specialized equipment, enabling wider use of protein interaction assays.

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Published on: August 12, 2019

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DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Array Technology

Background:

  • Protein microarrays enable miniaturized, multiplexed, interaction-based protein assays.
  • High costs and specialized equipment limit the widespread adoption of current protein microarray technologies.
  • Availability and stability of purified proteins are critical for microarray performance.

Purpose of the Study:

  • To develop an economical and accessible method for producing protein microarrays.
  • To overcome the limitations of conventional protein purification and microarray fabrication.
  • To enable on-demand printing of protein microarrays using cell-free protein synthesis.

Main Methods:

  • A novel DNA array to protein array technology was developed.
  • Reusable template DNA microarrays were used for repeated printing.
  • Cell-free protein synthesis was employed for on-demand protein production.
  • Macro-handling procedures replaced sophisticated microarraying apparatus.

Main Results:

  • Economical printing of protein microarrays from a reusable DNA template was achieved.
  • The method allows for on-demand production of protein microarrays.
  • Protein array fabrication became accessible without specialized equipment.
  • The cost and accessibility barriers for protein microarrays were significantly reduced.

Conclusions:

  • The developed technology offers a cost-effective and accessible approach to protein microarray production.
  • This method facilitates the broader application of protein interaction assays.
  • It democratizes protein microarray fabrication, making it feasible without specialized infrastructure.