Related Experiment Video
Updated: May 29, 2026
Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Published on: May 19, 2023
[Effect of BMP-2 on osteogenesis of bone mesenchymal stem cells in rats]
Jian Sun1, You-cheng Yu, Zhang-yu Gu
1Department of Stomatology, Fudan University. Shanghai, China. Sunjian225@hotmail.com
Purpose:
To determine the effect of bone morphogenetic protein-2(BMP-2) on osteogenesis of bone mesenchymal stem cells(BMSCs) in rats.
Methods:
The primary culture of rat BMSCs was succeeded and then was identified. The rat BMSCs were infected with the recombinant lentivirus with BMP-2(Lenti-BMP-2). The osteogenic effect of BMP-2 was observed. In addition, adhesive ability of BMP-2-BMSCs was detected through adhesion assay and expression of osteogenic factors OPN,OCN,Col-I and smad was observed by RT-PCR and Western blot. SPSS11.0 software package was used for analysis.
Results:
Rat BMSCs were cultured and identified successfully. The osteogenic effect of BMSCs was improved by BMP-2. Lenti-BMP-2 BMSCs adhesive potential enhanced and osteogenic factors were up-regulated.
Conclusions:
BMP-2 may facilitate the osteogenic effect of the rat BMSCs and would provide favourable cell resource for tissue engineering.
