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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Generation of Genetically Modified Mice through the Microinjection of Oocytes
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A method for the generation of conditional gene-targeted mice.

Masahiro Yamamoto1, Kiyoshi Takeda

  • 1Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|September 13, 2011
PubMed
Summary

This chapter details methods for creating knockout mice, essential tools for studying gene functions in vivo. These techniques enable the analysis of integrins and cell adhesion molecules in biological processes.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Immunology

Background:

  • Integrins and cell adhesion molecules are crucial for cell adhesion, movement, development, and immune responses.
  • Understanding their in vivo functions necessitates genetic manipulation in model organisms.
  • Conventional and conditional knockout mice are key tools for these investigations.

Purpose of the Study:

  • To describe methodologies for generating conventional and conditional knockout mice.
  • To provide insights into the technical procedures for gene targeting.
  • To facilitate the study of in vivo protein functions under physiological conditions.

Main Methods:

  • Gene targeting to create mice lacking specific molecules.
  • Development of targeting vectors for homologous recombination.
  • Selection of genetically modified embryonic stem cells.
  • Generation of both conventional and conditional knockout mouse models.

Main Results:

  • Established protocols for generating gene-targeted mice.
  • Demonstrated the utility of knockout mice for studying gene function in vivo.
  • Provided a comprehensive guide to the technical aspects of mouse model generation.

Conclusions:

  • Gene targeting is a powerful and widely used method for elucidating protein functions.
  • The described methods enable the creation of valuable mouse models for biological research.
  • These knockout mice are instrumental in understanding development and immune responses.