Related Experiment Video
Updated: May 29, 2026
08:05
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Bronchial allergen challenge using the Medicaid dosimeter.
Johannes Schulze1, Martin Rosewich, Melanie Dressler
1Department of Allergy, Pulmonology and Cystic Fibrosis, Children's Hospital, Goethe-University, Theodor Stern Kai 7, Frankfurt am Main, Germany. Johannes.Schulze@kgu.de
International Archives of Allergy and Immunology
|September 14, 2011
Summary
This study found that single-step allergen challenges are reproducible for measuring grass pollen allergy responses. This method is excellent for studying allergic airway inflammation and developing new treatments.
Area of Science:
- Allergy and immunology research
- Pulmonary medicine and respiratory diseases
- Asthma pathogenesis and treatment
Background:
- Bronchial allergen provocation is a key method in asthma research.
- Reproducibility of single-concentration, single-step allergen challenges was evaluated in grass pollen-allergic volunteers.
Purpose of the Study:
- To assess the reproducibility of single-concentration, single-step allergen challenges in grass pollen-allergic individuals.
- To determine the reliability of this method for studying allergen-induced airway inflammation.
Main Methods:
- Forty-seven subjects underwent incremental bronchial challenges to determine the provocative dose of 20% drop in FEV1 (PD20FEV1).
- Thirty-nine subjects then underwent repeated single-step challenges.
- Early and late asthmatic responses and exhaled nitric oxide levels were monitored.
Main Results:
- The mean maximal drop in FEV1 during the first single-step challenge was 21.3% ± 8.0.
- The second single-step challenge showed a mean FEV1 drop of 20.9% ± 7.2, with good intraclass correlation (0.37, p < 0.05).
- Increases in exhaled nitric oxide demonstrated substantial agreement between repeated challenges (ICC 0.62, p < 0.001).
Conclusions:
- Single-concentration, single-step allergen challenges using the aerosol provocation system are time-saving and less error-prone.
- This reproducible method is suitable for investigating mechanisms of allergen-induced airway inflammation.
- The findings support the use of this technique for developing novel treatments for allergic diseases.
Related Concept Videos
01:30Asthma-IV: Diagnostic and Management
The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
01:23Inhaled Medications
Inhaled medications are crucial for managing chronic obstructive pulmonary disease (COPD) and asthma. They are essential for effective treatment and control, ensuring optimal respiratory health and well-being. Inhaled medication delivers drugs directly to the lungs, providing a rapid onset of action and reducing systemic side effects compared to oral or injectable medications. Three primary types of inhalation devices are used to administer these medications: nebulizers, metered-dose inhalers...