Development of an enzyme linked immunosorbant assay for the detection of Cry2Ab Protein in transgenic plants

Immunoassays' a sensitive technique has a wide application for detection of antigen, especially for the detection of growing genetically modified crops, which are underwent field trial. Here, we have developed for the quantitative detection of Cry2Ab protein expressed in GM crops. A Cry2Ab-rabbit-IgG act as capture antibody and Cry2Ab mouse monoclonal antibody behave as a detecting antibody, which is employed in sandwich ELISA. A 2% polyvinylpyrrollidone and 1% dithiothritol was utilized in protein extraction from cotton seed to avert interfering agents. The developed assay was validated with GM cotton samples, limit of detection was found 1 pg/ml and range to a limit of quantification 16 pg/ml. The developed immunoassay does not show any cross reactivity with other non target GM proteins like Cry1Ab, Cry1Ac, EPSPS, Vip.